precision plus proteintm western c standards Search Results


precision plus proteintm unstained standards  (Bio-Rad)
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Bio-Rad precision plus proteintm unstained standards
Precision Plus Proteintm Unstained Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precision plus proteintm western c standards  (Bio-Rad)
99
Bio-Rad precision plus proteintm western c standards
Precision Plus Proteintm Western C Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kaleidoscope  (Bio-Rad)
96
Bio-Rad kaleidoscope
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
Kaleidoscope, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precision plus proteintm dual color standards  (Bio-Rad)
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Bio-Rad precision plus proteintm dual color standards
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
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precision plus proteintm western ctm standard  (Bio-Rad)
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Bio-Rad precision plus proteintm western ctm standard
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
Precision Plus Proteintm Western Ctm Standard, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sc 2003 precision plus proteintm dual color standards bio rad  (Bio-Rad)
97
Bio-Rad sc 2003 precision plus proteintm dual color standards bio rad
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
Sc 2003 Precision Plus Proteintm Dual Color Standards Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precision plus proteintm western ctm  (Bio-Rad)
96
Bio-Rad precision plus proteintm western ctm
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
Precision Plus Proteintm Western Ctm, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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molecular weight marker  (Bio-Rad)
96
Bio-Rad molecular weight marker
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
Molecular Weight Marker, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precision plus proteintm dual extra standards  (Bio-Rad)
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Bio-Rad precision plus proteintm dual extra standards
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
Precision Plus Proteintm Dual Extra Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precision proteintm streptactin hrp conjugate  (Bio-Rad)
94
Bio-Rad precision proteintm streptactin hrp conjugate
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
Precision Proteintm Streptactin Hrp Conjugate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mini proteantm tgx stain freetm protein gels  (Bio-Rad)
99
Bio-Rad mini proteantm tgx stain freetm protein gels
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
Mini Proteantm Tgx Stain Freetm Protein Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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proteontm glh sensor chip  (Bio-Rad)
93
Bio-Rad proteontm glh sensor chip
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
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Image Search Results


Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the Kaleidoscope ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.

Journal: Journal of Vaccines & Vaccination

Article Title: Immunization of Female Mice with a Plasmid DNA Vaccine Coding Eight Repeats of Gonadotrophin Releasing Hormone(Gnrh-I) and Eight T-Helper Epitopes Suppress Fertility In Vivo

doi: 10.4172/2157-7560.1000282

Figure Lengend Snippet: Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the Kaleidoscope ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.

Article Snippet: A Kaleidoscope (Precision Plus ProteinTM BIO-RAD) protein marker was included in the starting wells and the separated protein bands in the gels were transferred to a nitrocellulose membrane (HybondTMECL membrane, Amersham Bioscience).

Techniques: Western Blot, Transfection, Vaccines, Cell Culture, Negative Control, Control